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Figure 1: Deduce target S. mansoni sequence for the PCR amplification using the previously published primers (Sandoval, et al., 2006 a & b). Sequences of both the forward and reverse primers were compared with the published schistosome sequences on the GenBank (http://www.ncbi.nlm.nih.gov) using the basic nucleotide blast application for and results showed 100% homology to the highlighted termini of the below mentioned previously S. mansoni sequence suggesting their exact annealing positions on the parasite template. |