Table 2

Rhamnolipid	*Ionic species (m/z)**	Extra cellular amount† (10⁴ Area /OD₆₀₀)			Intra cellular amount‡ (10⁴ Area /OD₆₀₀)
Rhamnolipid	*Ionic species (m/z)**	H103	H636	H636O	H103	H636	H636O
Rha-C10-C10	503	12.3 ± 1.1	ND	11.3 ± 1.9	ND	ND	ND
Rha-C10-C12:1	529	3.9 ± 0.2	ND	2.2 ± 0.3	ND	ND	ND
Rha-C12-C10	531	2.9 ± 0.1	ND	2 ± 0.2	ND	ND	ND
Rha-Rha-C8-C10 Rha-Rha-C10-C8	621	32.5 ± 7.4	ND	24.9 ± 5.2	ND	ND	ND
Rha-Rha-C8-C12:1	647	3.1 ± 0.7	ND	1.9 ± 0.1	ND	ND	ND
Rha-Rha-C10-C10	649	502.4 ± 14.5	ND	321.7 ± 55.4	13.2 ± 6.3	5.1 ± 2.2	41.5 ± 12.8
Rha-Rha-C10-C12:1	675	138.1 ± 18.1	3.3 ± 0.3	113.8 ± 13.7	5.5 ± 2.2	1.5 ± 0.7	16.1 ± 5.5
Rha-Rha-C10-C12, Rha-Rha-C12-C10	677	662.9 ± 3.6	13.2 ± 1.5	257.5 ± 16.6	15.4 ± 6.3	5.6 ± 2.8	56.9 ± 19.4
Rha-Rha-C12-C12:1	703	15.6 ± 3.3	ND	12.7 ± 1.2	0.9 ± 0.5	0.8 ± 0.1	3.7 ± 1.4
Rha-Rha-C12-C12	705	42.9 ± 25.2	1.7 ± 0.2	18.7 ± 1.5	2.9± 0.2	1.7 ± 0.1	5.3 ± 1.7
Total amount of rhamnolipids		1416.7 ± 74.2	18.2 ± 2	766.8 ± 1.5	37.9 ± 15.5	14.7 ± 5.9	123.5 ± 40.8

Table 2: Rhamnolipid amounts produced by P. aeruginosa H103, H636 and H636O grown to stationary phase in LB medium. ND: values not detected. Threshold: <0.8 x 10⁴ Area/OD₆₀₀. *Ionic species leading to LC/MS peaks.† Rhamnolipids assayed from culture supernatants. ‡ Rhamnolipids assayed from cell pellets. †,‡ The LC/MS peak surface areas were divided by the OD₆₀₀ values of the cultures. Each value is the average of three independent experiments.