Figure 3: Transmigration of serotype 7F and corresponding capsule mutant through blood brain barrier models. Serotype7F wild type bacteria and 7F-isolate1Δcps were incubated with A) the ECV304-C6-BBB and B) the HBMEC-BBB for 6 h using an MOI of 10. Transmigration rates were determined by counting of CFU from the bottom well. Calculation of transmigration efficiency was performed based on the amount of positive transmigration events and presented as percentage from the total number of tested inserts. A total number of nine ECV304-C6-BBB transwells and of nine HBMEC-BBB transwells were used for calculation for each strain. Standard deviation represents triplicate data from two independent test series.