Figure 5: EhEBP1 binds to R9 motifs. (A) Western blot analysis of the partially purified R9(2) binding proteins. Lane 1, NE; lanes 2 and 3, partially purified fraction. Polypeptides were reveled using anti-EhEBP1 (lanes 1 and 2), and anti-C/EBPβ antibodies (lane 3). Arrowhead, polypeptides detected by the anti- EhBP1 antibody. (B) Supershift assay show binding of the EhEBP1 protein to labeled Pgp1-226/218R3 that specifically competed with an anti-EhEBP1 antibody (lane 3), but no by another heterologous antibodies against C/EBPβ transcription factor, (lane 4). Arrowhead, specific DNA-protein complex. (C) Comparative analysis of the URE4 sequences and the R9 motifs. Graphic representation of the URE4 and R9 nucleic acid sequences of the hgl5 and EhPgp1 gene promoters. Boxes sequences indicate the URE4 and R9 motifs and bold letters marked the differential bases in each sequence. Bases between each R9 and URE4 motifs are underlined.