![]() |
Figure 3: Gel electrophoresis separation of polymerase chain reaction products of multiplex Polymerase Chain Reaction typing of the genomic DNA of mycobacteria, isolated from milk and nasal swab samples of positive reactor animals run on 2% agarose gel. Lane: 1=a ladder of band at an interval of 100 bp DNA; 2=Mycobacterium avium-positive control; 3=Qiagene-water negative control; 4=Mycobacterium tuberculosis complex-positive control; 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16 and 17=positive samples for genus Mycobacterium; 8, 18 and 19=negative samples. |