Figure 5A and B: The Pan- genomic tree and 16S rRNA based tree comparison. The Pan-genomic tree (left) constructed, based on the presence and absence of conserved gene families in species (genomes) and 16S rRNA gene sequence homology based tree (right). In comparison, both the tree shares considerable similar pattern of genome distributions to clusters. According to Blast Matrix and pan-genomic calculations, the C. ulcerans and C. diphtheria, both pathogenic species are lies close to C. pseudotuberculosis (pathogens). The C. glutamicum non-pathogenic species clustered apart from pathogenic species based on both analyses. Hence, both strategies could be used in parallel for comparative analysis, typing and classification of bacterial species.