Isolate no. Inhibitory effect (%) Lytic enzyme activity (U/ml) Protease activity (U/ml)
Method no. 1 Method no. 2 Method no. 3
I1 61.5 ± 5.0 0.0 ± 0.0 75.0 ± 6.0 1.1 ± 0.10 448.1± 10.5
I2 75.0 ± 4.7 80.3 ± 2.1 93.8 ± 1.1 1.6 ± 0.12 524.2 ± 12.9
I3 47.5 ± 7.0 63.3 ± 5.0 94.8 ± 0.8 1.8 ± 0.11 616.0 ± 14.3
I4 61.3 ± 3.0 43.8 ± 8.0 92.0 ± 2.5 1.5 ± 0.14 492.1 ± 9.5
I5 (S. griseolus) 75.5 ± 3.2 80.8 ± 2.2 95.5 ± 1.0 2.0 ± 0.14 660.7 ± 13.7
I6 42.5 ± 10.0 0.0 ± 0.0 81.8 ± 3.0 1.3 ± 0.09 460.9 ± 8.9
I7 (S. vlavogriseus) 10.0 ± 6.2 0.0 ± 0.0 74.2 ± 7.2 1.4 ±0.10 470.0 ± 7.5
I8 5.5 ± 3.5 0.0 ± 0.0 47.3 ± 6.2 0.9 ± 0.09 264.0 ± 7.3
I9 (S. griseus) 62.8 ± 13.0 66.0 ± 6.0 90.5 ± 4.3 1.3 ± 0.07 520.8 ± 11.2
I0 42.5 ± 10.2 25.0 ± 5.0 76.8 ± 4.0 1.2 ± 0.11 488.6 ± 13.4
F value 33.779 229.570 37.709 29.847 270.123
P value 0.000 0.000 0.000 0.000 0.000
Table 1: Screening of actinomycetes isolates for ovicidal effect on Fasciola gigantica eggs by using three methods at 30°C. Data indicated that there is highly significant difference among all isolates at 0.01 levels (p ≤ 0.01).