Effect Protease activity (U/ml) Inhibitory effect (%) Crude enzyme (M1) 647.0 ± 5.0 95.1 ± 2.0 Crude enzyme (M2) 1030.5 ± 14.0 97.6 ± 1.5 Partially purified enzyme (M1) 718.6 ± 8.0 86.0 ± 3.0 Partially purified enzyme (M2) 1138.0 ± 5.2 89.9 ± 3.0 Dead enzyme (M1) 0.0 ± 0.0 19.5 ± 5.0 Dead enzyme (M2) 0.0 ± 0.0 25.5 ± 4.0 F value 8443.476 350.317 P value 0.000 0.000

Table 2: Comparison between the effect of crude, partially purified and dead enzyme on Fasciola gigantica eggs after 3 days of incubation at 30°C using S. griseolus . Data indicated that there is highly significant difference among all isolates at 0.01 levels (p ≤ 0.01). M1= medium no. 1. M2= medium no. 2