Groups GMT 0 days 7 days 14 days 21 days 28 days 35 days 42days 49 days 56 days Group-A - 181.0 362.0 512.0 512.0 512.0 362.0 362.0 362.0 Group-B - 181.0 362.0 512.0 512.0 512.0 362.0 362.0 362.0 Group-C - 128.0 256.0 362.0 362.0 362.0 256.0 256.0 256.0 Group-D - - - - - - - - -

Table 1: Geometric mean Antibody titer by Indirect Haemagglutination (IHA) test against E. bovis in calves vaccinated with sonicated sporocyst vaccine (un-challenged). Group A: Supernatant from sonicated sporulated oocyst; Group B: Sediment from sonicated sporulated oocyst; Group C: Un-sonicated sporulated oocysts; Group D: Non-vaccinated group