Groups Vaccinated Days post challenge 0 day 7 days 14 Days 7 days 14 days 21 days 28 days 35 days 42 days Group-A - - - - - 850 900 750 700 Group-B - - - - - 900 850 700 700 Group-C - - - - - 1050 950 900 850 Group-D - - - - - 20,000 24,050 20,050 18,050

Table 3: Post vaccination challenge response of calves (oocysts count). Group A: Supernatant from sonicated sporulated oocyst; Group B: Sediment from sonicated sporulated oocyst; Group C: Un-sonicated sporulated oocysts; Group D: Non-vaccinated group