Media used Growth Hours      Culture pH Opacity IOU (x109 org / ml) Average yield (Opacity IOU/ml) Purity Sterility Ex 1 Ex 2 Ex 3 Ex 1 Ex 2 Ex 3 B2 24 7.48 7.52 7.44 15 10 12 12.33 Passed Passed B2 36 7.81 7.88 7.79 30 25 28 27.66 Passed Passed B2 48 8.11 8.00 7.98 45 40 38 41.00 Passed Passed

Table 1a: Growth yield analysis of B. pertussis strain 509 at different growth hours in baffle mode Fermentor cultivation. Ex: Experiment, Org: Organisms, IOU: International opacity units.