S.No. Field isolate Light microscopy Gametocytaemia (%) Gametocyte stages PCR RT-PCR
    Parasitaemia (%) Gametocytes   I II III IV V 18S rRNA(P. falciparummonoinfections) Pfs25 Pfs25
1. RNC 52 1.50 + 0.7 + + + + - + + +
2. RNC 54 0.60 - 0.2 + + + + + + + +
3. RNC 55 0.70 - 0.5 + + + + + + + +
4. RNC 58 1.20 - 0.6 + + + + - + + +
5. RNC 59 1.0 - 0.5 + + + + + + + +
6. RNC 60 0.80 - 0 - - - - - + + +
7. RAP 4 0.65 - 0 - - - - - + - -
8. RAP 9 0.88 - 0.1 - - - + - + + +
9. RAP 10 0.85 - 0.6 + + + + - + + +
10. RAP 11 0.70 + 0.05 - - - + - + + +
11. RAP 14 1.32 - 0.2 - - - + + + + +
12. RAP 16 0.95 - 0.8 + + + + - + + +
13. NF54 1.2 - 0.9 + - + + + + + +
Table 2: Details of the P. falciparum isolates studied for gametocyte production and subsequently to analyze the Pfs25 gene expression levels.