| Pathogen |
Primer sequence (5'-3') |
PCR condition |
(Bp) |
| Theileria sp. |
F: AGTTTCTGACCTATCAG and R: TTGCCTTAAACTTCCTTG [27] |
95°C for 3 min., 33 cycle at 94°C for 30 sec., 50°C for 30 sec., 72°C for 30 sec. and final extension (f. ext.) at 72°C for 5 min. |
1100 |
| Theileria annulata |
F: ACT TTG GCC GTA ATG TTA AAC and
R: CTC TGG ACC AACTGTTTGG [28] |
95°C for 5 min., 33 cycle at 94°C for 30 sec., touchdown from 62°C for 30 sec., 72°C for 30 sec. and f. ext. at 72°C for 5 min. |
312 |
| Anaplasma marginale |
F: GCT CTA GCA GGT TAT GCG TC and
R: CTG CTT GGG AGA ATG CAC CT [29] |
95°C for 3 min., 35 cycle at 94°C for 30 sec., 57°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 7 min. |
265 |
| Babesia bovis |
F: TTTGGTATTTGTCTT GGTCAT and
R: ACCACTGTAGTC AAACTCACC [30] |
95°C for 3 min., 35 cycle at 94°C for 30 sec., 57°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 6 min. |
448 |
| Babesia bigemina |
F: TAG TTG TAT TTC AGC CTC GCG and
R: AAC ATC CAA GCA GCT AHT TAG [31] |
95°C for 3 min., 35 cycle at 94°C for 30 sec., 57°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 6 min. |
639 |
| Trypanosoma brucei |
F: GAA TAT TAA ACA ATG CGC AG and
R: CCA TTT ATT AGC TTT GTT GC [32] |
95°C for 3 min., 33 cycle at 94°C for 30 sec., 52°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 10 min. |
164 |
| Trypanosoma evansi |
F: GCG GGG TGT TTA AAG CAA TA and
R:ATT AGT GCT GCG TGT GTT CG [33]
|
95°C for 3 min., 33 cycle at 94°C for 30 sec., 52°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 10 min. |
205 |
| Borrellia burgdorferi |
F: CCT GTT ATC ATT CCG AAC ACA G and
R: TAC TCC ATT CGG TAA TCT TGG G [34] |
95°C for 3 min., 33 cycle at 94°C for 30 sec., touchdown from 62°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 5 min. |
411-452 |
| Rickettsia 16S rRNA |
F: GGG GGC CTG CTC ACG GCG G and
R: ATT GCA AAA AGT ACA GTG AAC A [35] |
95°C for 3 min., 33 cycle at 94°C for 30 sec., touchdown from 62°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 5 min. |
380 |
| Histophilus somni |
F: AGA GTT TGA TCA TGG CTC AG and
R: AGG GTT ACC TTG TTA CGA CTT [36] |
95°C for 3 min., 33 cycle at 94°C for 30 sec., 52°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 7 min. |
408 |
| Pasteurella multocida |
F: ATC CGC TAT TTA CCC AGT GG and
R: GCT GTA AAC GAA CTCGCCAC [37] |
95°C for 3 min., 35 cycle at 94°C for 30 sec., 50°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 10 min. |
460 |
| Mycoplasmaspp. |
F: GGGAGCAAACAGGATTAG AC CCT and R: TGCACCATC TGTCACT CTGTTAACC
TC [38] |
95°C for 3 min., 30 cycle at 94°C for 30 sec., touchdown from 70°C for 30 sec., 72°C for 30 sec. and f. ext.at 72°C for 5 min. |
270 |
