Figure 1: Viability and cultivability of the intracellular V. cholerae O1 tagged green fluorescent protein (GFP). A. Alone cultivated Acanthamoeba castellanii cyst emitting autoflourescene. B. Intracellular V. cholerae emitting green fluorescence. C. The intracellular V. cholerae grew on blood agar plate in presence of ampicillin. The colonies emitted green fluorescent when exposed to UV light.