Exp. No. A B C D E Dye Removal (%) Sample 1 Sample 2 Sample 3 L-1 5.0 25 25 Glucose Yeast extract 0 0 0 L-2 5.0 30 50 Starch Peptone 0 0 0 L-3 5.0 35 75 Sucrose NH4(NO3) 0 0 0 L-4 5.0 40 100 Lactose (NH4)2(SO4) 0 0 0 L-5 6.0 25 50 Sucrose (NH4)2(SO4) 0 0 0 L-6 6.0 30 25 Lactose NH4(NO3) 100 100 100 L-7 6.0 35 100 Glucose Peptone 0 0 0 L-8 6.0 40 75 Starch Yeast extract 0 0 0 L-9 7.0 25 75 Lactose Peptone 10 11 10 L-10 7.0 30 100 Sucrose Yeast extract 0 0 0 L-11 7.0 35 25 Starch (NH4)2(SO4) 41 39 40 L-12 7.0 40 50 Glucose NH4(NO3) 0 0 0 L-13 8.0 25 100 Starch NH4(NO3) 0 0 0 L-14 8.0 30 75 Glucose (NH4)2(SO4) 20 18 20 L-15 8.0 35 50 Lactose Yeast extract 30 32 30 L-16 8.0 40 25 Sucrose Peptone 21 20 19

Table 2: L-16 Orthogonal table as per Taguchi method for MG degradation.