Figure 4b: Western blot. Proteins from 2 mM IPTG induced recombinant E. coli cells carrying PR350 reacted to antibody raised against TCE inducible proteins extracted from PM102 strain, as shown in lane 3. Lane 2 shows that no reaction was obtained with proteins from recombinant E. coli cells carrying PR300, grown in presence of 2 mM IPTG. Lane 1 corresponding to the molecular weight marker also did not react to the antibody.