| Name |
Forward and reverse PCR primers (5’-3’) |
PCR product/primer features |
| R4F
R5R |
TTAAGAGCTCATGAGAGTAGCAAACGTAAG
TTAAACTAGTCCGGGTAACCCAGTTCC |
5’ ribosomal flanking sequence |
| R6F
R7R |
TTAACTCGAGGCACCTGTCACTTTGG
ATTAGGTACCATCAGGTTCCACCAAACAG |
3’ ribosomal flanking sequence |
| NEOF
NEOR |
AATTACTAGTATTTATGCAGAGGCCGAGG
TTATCTGCAGTCAGAAGAACTCGTCAAGAAG |
Neomycin resistance gene and promoter |
| CYC1F
CYC1R |
TTAACTCGAGATCCGCTCTAACCGAAAAGG
ATTTCTCGAGCTTCGAGCGTCCCAAAACC |
TcycI transcriptional terminator |
| GAPDHF
GAPDHR |
TTAACTGCAGCGAGTTTATCATTATCAATACTCG
TTAAAAGCTTCGAAACTAAGTTCTTGGTG |
GAPDH promoter |
| S308L-OPHF
S308L-OPHR |
CCAGAGTTTTTCGGAAGCAG
TCGGCTAACAGGGAAACATC |
S308L-OPH qPCR real time primers |
| YLR346CF
YLR346CR |
GGCTGTGCCTTTTCACTTTC
GGTAATGCGAATGAGCAGGT |
Internal qPCR real time control |
| OPHF
OPHR |
GAAGGCTTGTCGCCAAGTAG
GAGCCCTAGTCTGCCAACTG |
Internal OPH verification primers |
| S308L.INTF
S308L.INTR |
CAATCCCGCACGTTTCTTAT
AATGAGCCATTCGCAGTTTC |
Spanning 3’ S308L integration site |
| OPD.INTF
OPD.INTR |
GGAGGATAATGCATCGGCTA
TCCGTCACCATACCATAGCA |
Spanning 3’ OPH integration site |
Bold represents restriction endonuclease site sequences: GAGCTC, SacI; ACTAGT, SpeI; CTCGAG, XhoI; GGTACC, KpnI; CTGCAG, PstI; AAGCTT, HindIII.