Temperature (°C) Specific activityab Fold increase (compared to 4°C) 4 0.11×10-5 (0.09×10-5) N/A 22 0.71×10-5 (0.50×10-5) 6.5 30 3.40×10-5 (0.64×10-5) 30.9 37 4.80×10-5 (0.56×10-5) 43.6 aμmoles hydrolyzed/min/OD600 of 1 (after correction to control cultures) b average of three independent cultures (standard deviation) N/A, not applicable

Table 7: Specific activity of recombinant S308L-OPH yeast against demeton-S methyl incubated at different temperatures.