Experiments   Time(Days) Glucose (g/l) (NH4)2SO4 (g/l) pH COD0 (g/l) Inoculum size Agitation   Low level   4 0 0 5 10 107 -   High level   8 2 2 7 15 109 100rpm % color removal 1 + - - - + + + - 20 ±2 2 + + - - - - + + 32 ± 5 3 + - + - - + - + 50 ± 1.6 4 + + + - + - - - 48 ± 3 5 + - - + + - - + 30 ± 2.4 6 + + - + - + - - 19 ± 1.1 7 + - + + - - + - 44 ± 4 8 + + + + + + + + 53 ± 3.9

Table 2: 27-4 fractional factorial design with observed response values for decolorization of TOPW using Aspergillus niger.