Figure 11: a) The 10% native gel with protein fractions purified from culture supernatant. As enzyme activity was higher in the supernatant, extracellular proteins were purified by ammonium sulphate precipitation. At 80% ammonium sulphat e, one protein band of 13.9 KD gave activity a band was shown with arrows. b) The 10% native gel zymogram showing activity bands of the enzyme.