Run no. Factors H. Crude oil removal% L. Crude oil removal% Crude oil Conc.(w/v) SWD Conc.(w/v) Time(day) Observed Predicted Residual Cook’s distance Observed Predicted Residual Cook’s distance 1 1.00 0.50 17.50 91.80 90.04 1.180 0.134 99.12 98.08 1.367 0.723 2 0.63 0.50 17.50 92.06 92.72 -0.338 0.002 93.76 92.92 0.529 0.004 3 0.63 0.50 7.00 88.91 85.18 2.496 0.601 87.35 87.25 0.086 0.001 4 0.63 0.50 17.50 93.10 92.72 0.192 0.000 92.33 92.92 -0.376 0.002 5 1.00 0.20 7.00 73.38 75.06 -1.761 1.180 91.44 91.34 0.133 0.007 6 0.63 0.50 17.50 92.56 92.72 -0.083 0.000 91.52 92.92 -0.889 0.011 7 0.63 0.50 17.50 90.14 92.72 -1.315 0.023 92.65 92.92 -0.174 0.000 8 0.25 0.50 17.50 92.82 91.65 0.783 0.059 91.52 93.31 -1.492 0.215 9 0.25 0.80 28.00 97.05 96.13 0.975 0.368 94.38 94.78 -0.526 0.107 10 0.63 0.80 17.50 95.42 95.21 0.141 0.002 97.04 95.42 1.350 0.176 11 1.00 0.20 28.00 90.58 90.42 0.172 0.011 97.12 98.23 -1.446 0.795 12 0.63 0.50 17.50 90.75 92.72 -1.005 0.014 93.56 92.92 0.402 0.002 13 0.63 0.50 28.00 94.97 95.76 -0.532 0.027 94.09 92.96 0.945 0.086 14 0.25 0.20 7.00 75.80 76.61 -0.854 0.282 88.65 88.30 0.455 0.080 15 0.63 0.50 17.50 91.87 92.72 -0.434 0.003 91.25 92.92 -1.060 0.015 16 0.25 0.80 7.00 89.50 90.39 -0.938 0.341 91.02 90.22 1.053 0.429 17 0.63 0.20 17.50 89.06 86.34 1.823 0.320 94.76 95.14 -0.318 0.010 18 1.00 0.80 7.00 83.09 83.43 -0.361 0.050 93.81 95.17 -1.766 1.186 19 1.00 0.80 28.00 94.55 94.45 0.105 0.004 96.18 96.85 -0.868 0.287 20 0.25 0.20 28.00 86.29 86.68 -0.416 0.067 99.12 98.08 1.367 0.723

Table 2: Experimental matrix and results for Heavy and Light crude oil biodegradation.