Primer identification Nucleotide sequence (5’→3’) G+C content (%) ISSR 5 ACG ACGACGACG AC 64.29 ISSR 6 TTG TTGTTGTTGTTG GB 35.29 ISSR 9 GAT CGA TCG ATC GC 57.14 ISSR 10 CTT CTTCTTCTTCTT CCT CCTCCTCCTCCTCCT CT 51.43 17899A CAC ACA CAC ACA AG 50.00 17898B CAC ACA CAC ACA GT 50.00 UBC 825 ACA CAC ACA CAC ACA CT 47.06 UBC 827 ACA CAC ACA CAC ACA CG 52.94 UBC 841 GAA GGA GAG AGA GAG AYC 50.00 UBC 849 GTG TGT GTG TGT GTG TYA 44.44

Possible nucleotides for base B are C, G or T and for base Y are C or T.

Table 1: ISSR primers used for amplification of DNA from Betula papyrifera (white birch), Quercus rubra (red oak) and Acer rubrum (red maple) tree samples from eroded/disturbed, stable upland and reference sites from GSR.