Soil sampling during biodegradation Amount of soil collected from control and treated soil samples over the range of soil pH
HPLC analysis 1g per 1.5ml acetonitrile for extraction process
Microbial cfu count 1g per 9ml ringers solution for dilutions
Microbial activity 1g per 7ml extraction buffer
Soil enzyme activity 1g for Acid/Alkaline phosphates activity
3g for Manganese peroxidase/ Lignin peroxidase and laccase activity
2g for L-arginine ammonification and β-glucosidase activity
Table 1: Soil sampling for biodegradation activity.