Soil sampling during biodegradation |
Amount of soil collected from control and treated soil samples over the range of soil pH |
HPLC analysis |
1g per 1.5ml acetonitrile for extraction process |
Microbial cfu count |
1g per 9ml ringers solution for dilutions |
Microbial activity |
1g per 7ml extraction buffer |
Soil enzyme activity |
1g for Acid/Alkaline phosphates activity
3g for Manganese peroxidase/ Lignin peroxidase and laccase activity
2g for L-arginine ammonification and β-glucosidase activity |