| Soil enzyme |
Buffer set at pH 5.5, pH 7 and pH 8.5 |
Substrates |
References |
| Acid/Alkaline phosphatase |
Acid phosphate buffer/ sodium orthophosphate buffer containing NaN3 (sodium azide) |
25mM p-nitrophenyl phosphate oxidation |
[17] |
| β-Glucosidase |
50mM phosphate buffer |
1mM of p-nitrophenyl-β-D-glucopiranoside (ONPG) |
[22] |
| L-arginine ammonification |
0.5ml of an arginine solution (0.2%) |
L-arginine |
[23] |
| Manganese peroxidase (MnP) |
100µMol succinate lactate buffer (adjusted at pH 5.5, 7 and 8.5) |
0.1µM of 3-methyl-2-benzthaiazolinone hydrazone hydrochloride (MBTH), 5µl of 3-dimethylaminobenzoic acid (DMAB), 0.2µM of MnSO4and 0.1µM H2O2 |
[24] |
| Lignin peroxidase (LiP) |
50mM sodium acetate buffer along with 1% polyvinylpolpyrrolidone |
0.1µM of 3-methyl-2-benzthaiazolinone hydrazone hydrochloride (MBTH), 5µl of 3-dimethylaminobenzoic acid (DMAB), 0.2µM of MnSO4 |
procedure followed byfew modifications by D’Annibale et al. [22] |
| Laccase enzyme |
Buffer A containing (0.1M sodium acetate, 0.005 M CaCl2, 0.05% tween 80 and 1% polyvinylpolpyrolidone |
1mM of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) |
|
