Lysimeter Conditions Number of samples Heterotrophic cfu/g (geomean) YEPSS phenotypea cfu/g (geomean; % of positive samples) Quantitative PCR copies/g (% of positive samples) nahA tetA luxA 1 PAH contaminated + HK44 36 8904 78 (33%) NDb 4455 (17%) ND 2 PAH contaminated + HK44 36 14910 18 (61%) ND 872 (17%) 2052 (17%) 3 Not contaminated + HK44 36 870 10 (6%) ND ND ND 4 PAH contaminated + HK44 20 23924 49 (13%) ND ND ND 5 Not contaminated + HK44 21 957 7 (19%) ND ND ND 6 PAH contaminated without HK44 14 6189 65 (43%) ND ND ND

Table 3: Summary of measured parameters of soils sampled from six lysimeters after 14 years of inoculation with PAHs and P. fluorescens HK44.