Gram stain Spore position Motility Catalase Urease VP OPNG Nitrate Citrate Indole Gelatin Glucose Maltose Sucrose Lactose Manitol Growth (50%) PO GPR C - - - +   +   - + + + + +     PO1 GNR   +   -       - + - +   + + +   PO2 GPR S + + - + + + + -   +         + PO3 +=Positive Reaction; GPR=Gram Positive Rods; P=Vogues Proskaver; C=Central; PO=Probable Organism; PO2=Escherichia coli; -=Negative Reaction; GNR=Gram Negative Rods; OPNG=O-nitrophenyl-B-D-galactopyranoside; S=Subterminal; PO1=Clostridium sp.; PO2=Bacillus licheniformis.

Table 2: Microbial isolates and their physicochemical characteristics.