Figure 2: Production profile of phytase in optimized conditions in fermenter. The fermenter study for phytase production was carried out in a 7-l fermenter (Bioflo, New Brunswick Scientific, NJ, USA) with 5-l working volume, in optimized media containing (gl-1); mannitol 22.8, K2HPO4 5.18, Na2HPO4 3.25, and sodium phytate 9.68, respectively. The change in pH profile was also recorded during fermentation process. The incubation temperature was kept at 30°C.