Figure 1b: Detection of superoxide radicals (a reactive oxygen species; ROS) in Cd-treated fungal mycelia analyzed at the beginning and end of the treatment based on fluorescence microscopy. Red fluorescence due to reaction of the Superoxide radicals with dihydroethidium was the basis of detection. i-ii: Control filaments- one hour incubation; iii-iv: Cadmium (0.5 mM) - treated filaments - one hour incubation; v-vi: Control filaments - 72 hour incubation; vii-viii: Cadmium (0.5 mM) - treated filaments - 72 hour incubation.