Sequential base extracts (1 M KOH and 4 M KOH) were prepared from poplar and switchgrass biomasses, as described in Methods. The neutralized and dialyzed extracts were immobilized on the ELISA plates and then treated with either CjXyl10B or NpXyl11A as explained (see Methods). These xylanase-treated extracts were washed and then subjected to ELISA screening using a diverse collection of plant cell wall glycan-directed monoclonal antibodies (see Supplemental Table S1). Untreated base extracts were used as controls. The panel on the right hand side depicts the classes of McAbs that are specific to various plant cell wall glycans. The intensity of the McAb binding is depicted as a heatmap with a black-blue-red-yellow-white color scheme, with white depicting strongest binding and black depicting no binding. The white-dotted boxes highlight the xylan epitopes that are most prominently affected by the enzyme treatments in each extract.
