Figure 4: ELISA screening using cell wall glycan directed McAbs of various cell wall extracts generated from delignified poplar (A) and switchgrass (B) biomass before (controls) and after treatment with xylanases (experimental scheme shown in Figure 1).
The panel on the right hand side depicts classes of McAbs that are specific to various plant cell wall glycans. The intensity of the McAb binding was depicted as heat map with a black-blue-red-yellow-white color scheme with white color depicting strongest binding and black no binding. The green-dotted boxes highlight the removal of xylan epitopes in cell wall extracts generated from delignified poplar and switchgrass biomass after treatment with xylanases.