Figure 1: Cell cycle distribution of cells cultured on TCPS for 24 h at densities of 2 × 104 cells/cm2, 2 × 105 cells/cm2, and 4 × 104 cells/cm2. Black, gray, and white represents G0/G1, S and G2/M phases of cell cycle, respectively. Cell cycle phase was determined by flow cytometric analysis with PI staining of DNA. Data represent the average of three experiments.