A) a single cell (∼20 μm) is isolated and precisely released into
B) a 200 nl droplet of buffer solution;
c) sketch of the planar hydrophilic/hydrophobic structured glass slide with 1 μl PCR reaction mixture covered with 5 μl mineral oil.
|
| Figure 3: a) Microdissection and particle transfer via SPATS, integrated into
a conventional inverted optical microscope for single particle transfer to the
Lab-on-a-Chip (LOC): micrometer step motor providing exact positioning, the
carrier trunk with easy-to-fit click system, the sample adsorbing head and the
collection grid centered to a sample slide; b) workflow of the single particle
isolation using SPATS43: A) a single cell (∼20 μm) is isolated and precisely released into B) a 200 nl droplet of buffer solution; c) sketch of the planar hydrophilic/hydrophobic structured glass slide with 1 μl PCR reaction mixture covered with 5 μl mineral oil. |
