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Figure 4: Purification profile of DNA using an amino-coated μPF as a function of flow rate corresponding to an initial loading of (A) 250 ng, (B) 500 ng, (C) 1000 ng, (D) 2000 ng, (E) 3000 ng. For comparison, the performance of an uncoated μPF using 2000 ng of intial DNA loading is shown in (F). Columns represent (left to right) the initial, non-trapped, washed and eluted DNA fragments from μPF during purification process. |