Table 3

Gastrointestinal Compartments	Dose µg/g dry weight soil	Number of revertants/plate (mean ± SE) D
Saliva	0^B	314 ± 2^E
	0^C	450 ± 39
	1	477 ± 4
	10	646 ± 11 ^F
	50	950 ± 0 ^F
Positive Control^A		1073 ± 5 ^F
Gastric	0^B	314 ± 2 ^E
	0^C	390 ± 7
	1	394 ± 4
	10	417 ± 2
	50	381 ± 9
Positive Control^A		1073 ± 5 ^F
Duodenal + Bile	0^B	314 ± 2 ^E
	0^C	356 ± 5
	1	374 ± 60
	10	687 ± 101 ^F
	50	956 ± 0 ^F
Positive Control^A		1073 ± 5 ^F
Sequential	0^B	314 ± 2^F
	0^C	393 ± 6
	1	419 ± 4
	10	428 ± 4
	50	440 ± 15
Positive Control^A		1073 ± 5 ^F

^APositive Control for TA102-Mitomycin C (0.5 μg/plate).
^BNegative control: Filter sterilised water.
^C Negative control for each gastrointestinal compartment (i.e. saliva, gastric, duodenal and sequential). These samples contained un-amended soils (soils without MMC).
^D Number of histidine revertants per plate: Mean values of at least three plates ± standard error (SE).
^E Numbers in italic, boldface and underlined represent the number of spontaneous revertants colonies for TA102 (117-530).
^F Numbers in boldface represent two fold increase or more in the number of revertant colonies over the solvent controls (spontaneous revertants), which was an indication of a significant mutagenic response.

Table 3: The number of reverse mutants of S. typhimurium TA102 after exposure to Insch soils amended with MMC and extracted by the sequential and compartmental (saliva, gastric and duodenal) in vitro extraction.