Figure 3: Determination of cell seeding in 3D liver cell model using reflection coefficient S11 (dB). 0, 0.25 x106, 0.5x106 and 1x106 HepaRG cells are grown within scaffold for 24 h. A) Reflection coefficient profiles S11 of 3D liver cell models consisting of different cell numbers. Plot of S11 (dB) versus frequency (HZ), measured with a network analyzer. Cell seeding was determined at frequency f = 73 MHz. B) Data analysis of reflection coefficient S11 measurement at 73 MHz, control (scaffold without cells) was set at 100%, and C) Reference curve and linear regression, SEM ± 3-4.