PMA treatment distinguishes viable from

Organism	Viability	Untreated C_T (SD)	LED CT (SD)	LED + PMA C_T (SD)	ΔC_T viable or ΔC_T nonviable	ΔC_T
B. anthracisSterne spores	Nonviable	31.87 (0.30)	32.17 (0.22)	ND	N/A	N/A
B. anthracisSterne spores	Viable	33.67 (0.16)	34.25 (0.31)	ND	N/A	N/A
B. anthracisSterne Vegetative	Nonviable	21.76 (0.07)	21.80 (0.11)	34.15 (0.40)	-12.4	-7.2
B. anthracisSterne Vegetative	Viable	21.66(0.03)	21.87 (0.18)	26.90 (0.24)	-5.24	-7.2
Y. pestispgm-	Non-viable	16.1 (0.06)	16.1 (0.17)	27.5 (1.03)	-11.4	-6.6
Y. pestispgm-	Viable	16.6 (1.47)	16.6 (1.46)	21.4 (1.19)	-4.8	-6.6

Table 2: PMA treatment distinguishes viable from nonviable B. anthracis Sterne vegetative cells and Y. pestis pgm- cells but not B. anthracis spores. Raw CT values of of viable and nonviable samples. The PMA concentration is 10 μM for B. anthracis Sterne spores and vegetative cells and 25 μM for Y. pestis pgmsamples. Spore samples contained 15,000 spores. ND, absence of qPCR signal. N/A, not applicable. Measurements are the average value of three independent e × periments. All ΔC_T values are calculated using equations (1), (2), or (3) as described in Materials and Methods.