Figure 4: (A) Effect of temperature on the interaction of BoNT/A antigen with the immobilized BoNT/A antibody on carboxymethyldextran modified gold chip. Temperature range: 10 °C-37 °C, pH: 7.5 and BoNT/A antigen concentration: 4.5 fM and (B) Effect of pH on the interaction of BoNT/A antigen with the immobilized BoNT/A antibody. pH range: 4.0-9.0, temperature: 25 °C and concentration: 4.5 fM.