Bacterial Species Strain ID Additional Information pXO1-MGB PCR pXO2-MGB PCR Ba chr-MGB PCR B. anthracis LSU 34   +   + B. anthracis LSU 39   +   + B. anthracis LSU 62 pXO1- -   + B. anthracis LSU 102   +   + B. anthracis LSU 149   +   + B. anthracis LSU 158   +   + B. anthracis LSU 174   +   + B. anthracis LSU 188   +   + B. anthracis LSU 193   +   + B. anthracis LSU 248   +   + B. anthracis LSU 256   +   + B. anthracis LSU 264   +   + B. anthracis LSU 267 V770-NP1-R; pXO2- +   + B. anthracis LSU 293   +   + B. anthracis LSU 328   +   + B. anthracis LSU 379   +   + B. anthracis LSU 419   +   + B. anthracis LSU 442   +   + B. anthracis LSU 462 Ames +   + B. anthracis LSU 463   +   + B. anthracis LSU 465   +   + B. anthracis LSU 488 Vollum +   + B. anthracis LSU 489   +   NT B. anthracis 4599 Scottish heroin strain +   + B. anthracis UM23 CL2 pXO1-; pXO2- - - + B. circulans NCTC 2610   - - - B. cereus NCTC 6474   - - - B. cereus NCTC 7464   - - - B. cereus NCTC 8035   - - - B. cereus NCTC 9939   - - - B. cereus NCTC 9945   - - - B. cereus NCTC 9946   - - - B. cereus S2-8   - - - B. cereus 3A   - - - B. cereus D17   - - - B. cereus F1-17   - - - B. cereus ATCC 10876   - - - B. cereus NCTC 11145   - - - B. cereus NCTC 10320   - - - B. cereus NCTC 11143   - - - B. coagulans NCTC 10334   - - - Brevibacillus brevis NCTC 2611   - - - B. atrophaeus NCTC 10073   - - - B. atrophaeus ATCC 51189   - - - B. atrophaeus Novozyme   - - - B. atrophaeus ATCC 9372   - - - B. licheniformis NCTC 10341   - - - B. mycoides NCTC 9680   - - - B. pasteurii NCTC 4822   - - - B. thurigiensis Al-Hakam   - - - B. thurigiensis var. kurstaki   - - - B. thurigiensis HD-571   - - - B. thurigiensis 97-27   - - - a(+) positive PCR result; (-) negative PCR result; (NT)–not tested

Table 1: B. anthracis strains, and close relatives, tested by the real-time PCRs developed in this study.