| Spore Dilution |
CFU/mL1 |
CFU/filter cup2 |
T0 Ct3 |
Ave (SD) T9 Ct |
Ave. ΔCt |
| 10-3 |
3.6E8 |
1.4E7 |
NDT |
24.1 (1.0) |
20.9 |
| 10-4 |
3.6E7 |
1.4E6 |
NDT |
24.8 (1.5) |
20.2 |
| 10-5 |
3.6E6 |
1.4E5 |
NDT |
25.7 (0.6) |
19.3 |
| 10-6 |
3.6E5 |
1.4E4 |
NDT |
27.0 (2.2) |
18.0 |
| 10-9 |
360 |
14 |
NDT |
28.5 (1.4) |
16.5 |
| 10-10 |
36 |
1.4 |
NDT |
33.1 (1.7) |
11.9 |
| 10-6 Killed4 |
12 |
4.7 |
NDT |
34.4 (0.6) |
10.6 |
*The RV-PCR protocol was according to that described in the Materials and
Methods section except that 13 mL instead of 14 mL extraction buffer were
removed at each extraction step.
1The CFU/mL value was determined from plate counts of the CRP BG spore dilutions.
In each case, 100 μL of the appropriate spore solution was added to the wipe.
2The CFU/filter cup values were calculated by dividing the CFU/mL by 10 since 100
μL was added, then dividing by 33 since the total extraction buffer volume was 33
mL, and finally, multiplying by 13, since 13 mL of extraction buffer was added to
the filter cup.
3NDT = Non-detect; The Ct (T0) was set to 45 to calculate ΔCt.
4Plate counts showed that complete kill was not achieved. |
