Figure 3: Growth rate in different cryopreservation solutions. A) Image of cultured cells after storage. B) Relative adherent cell rates. Human adipose tissues were preserved at −180°C for 7 days in one of three cryopreservation solutions: 10% DMSO + 90% FBS; 10% DMSO + 90% human plasma; and 10% DMSO + 10% FBS + 80% DMEM. Cells were cultured in media (DMEM containing 10% FBS) for 7 days. For the relative adherent cell rate, adherent cells from tissues cryopreserved with 10% DMSO + 90% FBS was treated as 100%. The relative adherent cell rate was determined by counting cells stained with 0.4% trypan blue dye. Scale bar = 40×, 100 μm.