Figure 1: a) the pollen in anthers were in early-uninucleate stage in R.retusa; b) pollen in late-uninucleate stage; c) the initiation of callus in Sharma medium; d, e) the callus was achlophyllous, compact & irregular in shape; f) two plbs formed in BAP &NAA(1mg/l); g) Acclimatized plantlet.