S.NO CONSTITUENTS Sharma medium (mg/l) 1 Macronutrient Ca(NO3)2.4H2O 200 2 KNO3 180 3 (NH4)2.SO4 100 4 NaH2PO4. 2H2O 150 5 MgSO4.7 H2O 250 6 Micronutrient KI 0.03 7 MnCl2. 4H2O 0.4 8 ZnSO4. 7 H2O 0.05 9 H3BO3 0.6 10 CuSO4.5 H2O 2.2 11 Na2MoO4 0.05 12 CO(NO3)2.6H2O 0.05 13 MnSO4. 7 H2O   14 CoCl2 6 H2O   15 Na2FeEDTA Separate stock solution of Na2FeEDTA is preparedby disolving 0.745g of Na2EDTA and 0.557g of FeSO4in100ml ofdistilled water.3 ml/l of this solution is used. 16 Vitamins Thiamine - HCL 0.3 17 Pyridoxine - HCL 0.3 18 Nicotinic acid 1.25 19 Riboflavin 0.05 20 Biotin 0.05 21 Folic acid 0.3 22 Sucrose 20 g/l 23 Agar 9 g/l 24 BAP 10 mg/l 25 NAA 1 mg/l 26 Peptone 2 mg/l

Table 2: Composition of Induction basal media commonly used in Orchids anther culture.