Figure 4: Coomassie blue stained SDS-PAGE patterns of PEGase1 expressed by E. coli Origami (A), and T. reesei QM9414 (B). Lane M in A and B: the standard MW Protein marker; lanes 1 and 2 in A: the lysates of pET32a- and pET32-pegase1-containing E. coli transformants; lanes 1~3 in B: the culture supernatant of the control mold (lane 1) and its pegase1 gene-containing fungus after being cultured for 2 and 5 days (lanes 2 and 3); the arrowed bands in lane 2 of A at 51 kDa, lanes 1~3 of B at 66 kDa are for Trx-PEGase1 and PEGase1, respectively.