Donor   [[α]1hl/u]-RT1.Aa   Low dose CsA  (3 days) High dose CsA (7 days)   Primed T cells   Naïve T cells   Total T cells   CD4+  T cells   CD8+ T cells   # of Transferred T cells (secondary recipient)   Mean Survival Time WF - - - + - - - - 1x107 6 days WF - - - - + - - - 1x107 14.4 days WF - - + - - + - - 1x107 >100 days WF + + - - - + - - 1x107 >100 days WF - - + - - - + - 1x107 >100 days WF + + - - - - + - 1x107 >100 days WF - - + - - - - + 1x107 87 days WF + + - - - - - + 1x107 28 days Primary recipients of WF grafts were conditioned with allochimeric class I MHC, [[α]1hl/u]-RT1.Aa (1mg/kg), in addition to 3 days of sub-therapeutic CsA (10mg/kg) or 7 days of high dose CsA alone (10mg/kg). Secondary recipients transplanted with WF grafts then received 1x107 T cells (total, CD4+, or CD8+), peri-operatively, isolated from a naïve animal or from primary recipients that were either untreated (primed T cells) or conditioned with CsA or CsA+ allochimeric class I MHC (CsA+P). Table shows expected decreased graft survival in primed and naïve T cells treated groups. There was no difference in graft survival between animals receiving CsA+P - compared to those that received CsA alone - conditioned cells, nor was there a difference in those that received CD4+ cells. Survival was significantly less in secondary recipients that had received CD8+ T cells conditioned with CsA+P.

Table 1: Allograft survival following adoptive transfer after class I MHC or high dose CsA conditioning.