Figure 2: Treatment of apoptotic T cells modulates production of IL-12 and IL-10 in DCs. Splenocytes were isolated from C57 BL/6J mice and stained with CD11c antibody. CD11c+ cells (DCs) were sorted by FACS. CD11c+ DCs were incubated with apoptotic or fresh T cells as a control. Expression of IL-10 (A) and IL-12 (B) in CD11c+ DC is shown. Isotype controls are also demonstrated. Error bars represent mean and SD of triplicate determinations of percentage (%) of cytokine expression in CD11c+ DCs in three independent experiments (n=3, t test, *P< 0.05).