Figure 4: Localization of IRAK-4 in microglia and astrocytes by double-immunofluorescence staining. (A-C) Immunofluorescent staining of CD68 (A, green) and IRAK-4 (B, red) in the temporal cortex of a representative AD case (higher magnification depicted in A1-C1). (D-F) Double staining for GFAP (D, green) and IRAK-4 (E, red) in the temporal cortex of a representative AD case (arrows indicate double-labelling, higher magnification depicted in D1-F1). Scale bar A-F: 50 μm; scale bar A1-F1: 10 μm.