Figure 5: MCP-1 and IL-6 secretion by human primary astrocytes in the presence of IRAK-1/4 inhibitor I. (A) IL-1β (10 U/ml) induced secretion of MCP-1 (upper panel) and IL-6 (lower panel) after 24 hours detected in the supernatant of cultured human primary astrocytes in the presence of IRAK inhibitor I (250, 2500, and 5000 nM). (B) Effects of IRAK inhibitor I on the IL-1β (10 U/ml) induced secretion of MCP-1 as detected in the culture supernatant of human astrocytes after 6 and 24 hours incubation in the presence of 2500 nM IRAK inhibitor I. Results are expressed as mean (± SD) of one representative experiment assayed in triplicate. Asterisks indicate significant difference compared with IL-1β treatment without IRAK inhibitor I treatment (p<0.05).