Figure 1: The frequency of FoxP3+ Tregs is higher in HA+ fraction compared to HA- fraction of CD4+ cells isolated from both healthy donors and breast cancer patients. CD4+ cells obtained from peripheral blood were magnetically separated into HA+ and HAfractions. The frequency of HA+ and HA- cells in CD4+ was determined (A). The percentage of CD4+CD25+FoxP3+ (B) and CD4+FoxP3+ (C) Tregs in HA+ and HA- fractions was analyzed; paired data and medians for each group of variables are represented.