Fraction Total proteina ( µg×104) Activity unitb (Au ×106 ) Specific activityc (Au/µg×102) Purification fold Yield (%) Crude ES 29.2 7.3 0.25 1.00 100.00 Unbound to column 16.3 0.5 0.031 0.22 6.85 Bound and eluted fraction 0.19 6.4 33.53 2051.5 87.67

Table 1: Quantitative summary of F. gigantica ESPs purification. aProtein was measured as described by Lowry et al. [22]. bA unit of activity defined as the amount of protein required to give one well of agglutination. cSpecific activity which is the number of activity per µg of protein and is related to the starting crude ESPs.