Figure 5: Effect of monocyte co-cultures and CCL2 on PC-3 High Invasive prostate cancer cell invasion. (A) PC-3 High Invasive cell invasionwas induced by co-cultures with U-937 or THP-1 cells and was inhibited by anti-CCL2 neutralizing antibodies compared with isotype controlantibodies. (B) PC-3 High Invasive cell invasion was induced by addition of purified recombinant human CCL2 protein to the lowerTranswell chamber with optimum at 10 ng/mL CCL2. (C) PC-3 invasion activity was induced by addition of 10 ng/mL CCL2 or 5 ng/mLinterleukin-8 to a similar degree as induced by U-937 co-cultures. The percent PC-3 cell invasion activity is expressed as mean ± SD of 3independent experiments. *P<0.05; **P<0.001.